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EC Number	Protein Variants	Commentary	Reference
4.1.99.22	R266A	complete loss of activity	716738
4.1.99.22	R268A	complete loss of activity	716738
4.1.99.22	R71A	80% loss of activity	716738
4.1.99.22	R88Q	mutant xd22 has a mutation in CNX2 affecting the proximal FeS cluster loop. Complementation of the cnx2-2 phenotype by a genomic fragment including the promoter region from position -1224 and UTRs, amplified by PCR. Mutant cnx2-2 plants are transformed using Agrobacterium tumefaciens strain GV3101. The cnx2-2 mutation is not a knockout allele and growth is rescued by ammonia. Crosses between heterozygous cnx2-1 CNX2 and homozygous cnx2-2 plants give F1 offspring in two phenotype categories: seedlings with wild-type appearance corresponding to cnx2-1 CNX2 and small chlorotic seedlings that are genotyped as cnx2-1 cnx2-2	-, 747034
4.1.99.22	S126A	mutant enzyme with low activity	716738
4.1.99.22	T73A	mutant enzyme with low activity	716738
4.1.99.22	Y30A	mutant enzyme with low activity	716738

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Release 2023.1 (January 2023)