EC Number |
Protein Variants |
Reference |
---|
3.6.4.6 | E233Q |
no ATP hydrolysis, enhanced binding to Vps20p and Vta1p in vitro |
656345 |
3.6.4.6 | E233Q |
site-directed mutagenesis, an ATPase inactive mutant |
734303 |
3.6.4.6 | E235Q |
no ATPase activity, mutation leads to perturbation of various membrane transports via endosomes |
656346 |
3.6.4.6 | E243A |
site-directed mutagenesis, a pore loop mutant, displays essentially unchanged oligomerization, introducing E243A point mutation increases the affinity of peptide C binding by 3fold, shows reduced ATPase activity compared to the wild-type |
734303 |
3.6.4.6 | E243A/E247A |
site-directed mutagenesis, pore loop mutant, displays essentially unchanged oligomerization, the double mutant binds peptides 4fold (peptide C) or 10fold (peptide B) more tightly than wild-type Vps4p, shows reduced ATPase activity compared to the wild-type |
734303 |
3.6.4.6 | E247A |
site-directed mutagenesis, a pore loop mutant, displays essentially unchanged oligomerization, introducing E247A point mutation increases the affinity of peptide C binding by 4fold, shows reduced ATPase activity compared to the wild-type |
734303 |
3.6.4.6 | E305Q |
mutant assembles into oligomer like wild-type, similar ATPase activity as wild-type |
656763 |
3.6.4.6 | E305Q/E578Q |
no ATPase activity |
656763 |
3.6.4.6 | E329Q |
hydrolysis mutant |
686724 |
3.6.4.6 | E329Q |
in contrast to wild-type NFS E329Q mutant is enriched on various structures throughout the cell and is only minimally released by saponin permeabilization |
656763 |