EC Number   |
Protein Variants |
Reference |
|---|
   3.5.5.5 | Tyr54C |
the mutant shows wild type activity but forms significantly decreased relative amounts of atrolactamide from 2-hydroxy-2-phenylpropionitrile |
718648 |
| 3.5.5.5 | Tyr54P |
the mutant shows wild type activity but forms significantly decreased relative amounts of atrolactamide from 2-hydroxy-2-phenylpropionitrile |
718648 |
| 3.5.5.5 | Tyr54V |
the mutant shows wild type activity but forms significantly decreased relative amounts of atrolactamide from 2-hydroxy-2-phenylpropionitrile |
718648 |
| 3.5.5.5 | W110F |
the mutant shows activity similar to the wild type enzyme |
710940 |
| 3.5.5.5 | W163A |
the mutant exhibits a decreased activity for (R,S)-2-phenylpropionitrile compared to the wild type enzyme |
-, 734496 |
| 3.5.5.5 | W168A |
the mutant forms significantly increases amounts of mandelamide and 2-phenylpropionamide and demonstrates an almost complete inversion of enantioselectivity for the conversion of (R,S)-2-phenylpropionitrile (from R- to S-selectivity) |
-, 734496 |
| 3.5.5.5 | W188K |
the mutant enzyme produces almost exclusively phenylglycine amide from (R,S)-phenylglycinonitrile. In comparison to the wild type, there is an increase in the ee-value of the formed (S)-phenylglycine amide |
-, 755880 |
| 3.5.5.5 | Y141A |
the mutant enzyme is still active and converted the aliphatic (valeronitrile) and the aromatic substrate (2-phenylpropionitrile) with similar relative activities as the wild-type enzyme |
-, 758540 |
| 3.5.5.5 | Y141W |
the mutant enzyme converts valeronitrile with a much higher relative activity than 2-phenylpropionitrile |
-, 758540 |
| 3.5.5.5 | Y54A |
the mutant shows wild type activity but forms significantly decreased relative amounts of atrolactamide from 2-hydroxy-2-phenylpropionitrile |
-, 718648 |
