EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.97 | H61A |
mutation impairs protease activity. Morphology of mutant capsids exclusively resembles those of procapsids or large cored B capsids. Specifically, mutant capsids are spherical and lack electron dense cores. Most of these particles are tightly clustered in the nucleoplasm. Mutant does not replicate to appreciable extents on CV1 cells |
732971 |
| 3.4.21.97 | more |
IC-assemblin and IC-assemblinHis mutants of assemblin, blocked for internal and cryptic site cleavage. Enzymatic activities of pPR mutants are indistinguishable from that of IC-assemblin |
681686 |
| 3.4.21.97 | more |
stabilized against self-cleavage by a single point mutation in its cleavage site (IC-assemblin) |
718057 |
| 3.4.21.97 | S118A |
inactive mutant, active if coexpressed with AW5 which encodes the first 179 amino acids of assemblin with the addition of Ile, Gln, Thr |
95597 |
| 3.4.21.97 | S132A |
active-site mutant |
95603 |
| 3.4.21.97 | S132A |
is inactive |
677592 |
| 3.4.21.97 | S132A |
mutant enzyme is catalytically inactive |
650245 |
| 3.4.21.97 | S134A/A143Q |
the ratio of turnover number to Km-value is 150fold lower than that of the wild-typelike mutant enzyme A143Q |
650071 |
| 3.4.21.97 | S134A/A143Q/H157A |
the ratio of turnover number to Km-value is 174fold lower than that of the wild-typelike mutant enzyme A143Q |
650071 |
| 3.4.21.97 | V141G/V207G |
soluble and stable, indistinguishable from wild-type in vitro |
95604 |
