EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.47 | more |
enzyme component C3 deletion mutant. Acute lung inflammatory injury is greatly attenuated in lungs of mutant animals, and C5a levels in broncheolar lavage fluids of mutants greatly reduced in presence of antithrombin III or hirudin, compared to wild-type. Plasma from mutant animals contains threefold higher levels of thrombin activity and higher levels of prothrombin mRNA, prothrombin and thrombin protein in liver of mutants |
670361 |
| 3.4.21.47 | more |
introduction of Cys-residues to form a disulfide bond at positions 428 and 435 of von Willebrandt factor type A domain of subunit Bb. Adaption of an active conformation by the domain, which is not sufficient to activate the enzyme catalytic apparatus |
670220 |
| 3.4.21.47 | more |
replacement of C-terminal sequences of enzyme compoment C3 by the corresponding sequences of cobra venom factor from Naja kaouthia. Major role of NTR/C345C motiv in regulation of enzyme half-life |
668899 |
| 3.4.21.47 | more |
substitution of enzyme component C3 domains by corresponding domains of cobra venom factor. Structural domains of cobra venom factor gamma- or beta-chains confer stability to C3 convertase complex. Replacement of terminal C-275 amino acids by those of cobra venom factor beta-chain results in a catalytic activity of C3 convertase similar to wild-type, but with a half-life of 5-6 h compared to 1-2 min for wild-type |
669714 |
| 3.4.21.47 | N260D |
mutation in the Bb component decreases sensitivity to DAF to 32% of the wild-type value, sensitivity to CR1 to 31% of the wild-type value and sensitivity to Factor H to 24% of the wild-type value |
652225 |
| 3.4.21.47 | Q335A |
mutation in the Bb component decreases sensitivity to DAF to 20% of the wild-type value |
652225 |
| 3.4.21.47 | S339A |
mutation in the Bb component decreases sensitivity to DAF to 32% of the wild-type value, sensitivity to CR1 to 50% of the wild-type value |
652225 |
| 3.4.21.47 | W343A |
mutation in the Bb component decreases sensitivity to DAF to 55% of the wild-type value, sensitivity to CR1 to 56% of the wild-type value |
652225 |
| 3.4.21.47 | Y338A |
mutation in the Bb component decreases sensitivity to DAF to 9% of the wild-type value, sensitivity to CR1 to 4% of the wild-type value and sensitivity to Factor H to 48% of the wild-type value |
652225 |
| 3.4.21.47 | Y338F |
mutation in the Bb component decreases sensitivity to DAF to 18% of the wild-type value, sensitivity to CR1 to 20% of the wild-type value and sensitivity to Factor H to 61% of the wild-type value |
652225 |
