EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.9 | S146G |
site-directed mutagenesis, low remaining activity |
637580 |
| 2.5.1.9 | S41A |
site-directed mutagenesis, mutant produces a dimeric pentacyclic reaction intermediate, i.e. compound Q, which can be cleaved in 2 different ways by the enzyme |
657905 |
| 2.5.1.9 | S41A |
site-directed mutagenesis, very low remaining activity |
637580 |
| 2.5.1.9 | T3R |
site-directed mutagenesis, slightly reduced activity, low expression rate |
637580 |
| 2.5.1.9 | T50A |
production by site-directed mutagenesis, replacement of threonine residue with alanine decreases the acidity of protein-bound by 1-2 orders of magnitude |
704202 |
| 2.5.1.9 | T67A |
production by site-directed mutagenesis, replacement of threonine residue with alanine decreases the acidity of protein-bound by 1-2 orders of magnitude |
704202 |
| 2.5.1.9 | T71A |
site-directed mutagenesis, slightly reduced activity |
637580 |
| 2.5.1.9 | W207A |
the mutant enzyme is taken up by lumazine synthase only 30% less efficiently than wild-type enzyme |
759481 |
| 2.5.1.9 | Y133A |
site-directed mutagenesis, soluble protein, too unstable to be purified |
637580 |
