EC Number   |
Protein Variants |
Reference  |
|---|
   1.7.1.13 | C190A |
mutation in catalytic residue, no evidence of covalent binding of substrate preQ0. Mutant displays proton uptake |
742900 |
| 1.7.1.13 | C190A |
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity |
764418 |
| 1.7.1.13 | C190S |
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity |
764418 |
| 1.7.1.13 | D197A |
the variant does not take up protons in conjunction with preQ0 binding |
764418 |
| 1.7.1.13 | D197H |
the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme |
764418 |
| 1.7.1.13 | H229A |
the variant readily forms the thioimidate adduct and is 24fold less active for preQ0 reduction than wild type enzyme |
764418 |
| 1.7.1.13 | E89A |
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product |
765048 |
| 1.7.1.13 | E89L |
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product |
765048 |
| 1.7.1.13 | F228A |
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product |
765048 |
| 1.7.1.13 | I192A |
the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 1:1 ratio |
765048 |
