EC Number   |
Protein Variants |
Reference |
|---|
    1.2.7.11 | S41A |
the mutant shows reduced activity compared to the wild type enzyme |
-, 739701 |
| 1.2.7.11 | T256A |
kcat and Km for 2-oxoglutarate are 33% and 51%, respectively, as compared with that of the wild-type enzyme |
-, 719399 |
| 1.2.7.11 | T256A |
kcat/Km for pyruvate is 21% of wild-type value, kcat/KM for 2-oxoglutarate is 15% of wild-type value |
-, 719399 |
| 1.2.7.11 | T256S |
kcat/Km for pyruvate is 15% of wild-type value, kcat/KM for 2-oxoglutarate is 92% of wild-type value |
-, 719399 |
| 1.2.7.11 | T256V |
kcat/Km for pyruvate is 17% of wild-type value, kcat/KM for 2-oxoglutarate is 68% of wild-type value |
-, 719399 |
| 1.2.7.11 | T349L |
mutant enzyme StOFOR1 with mutation T349L in alpha-subunit. Vmax with pyruvate as substrate is 43% compared to wild-type enzyme, Vmax with 2-oxoglutarate as substrate is 74% compared to wild-type enzyme, Km with pyruvate as substrate is 1.6fold higher as compared to wild-type enzyme, Km with 2-oxoglutarate as substrate is 1.1fold higher as compared to wild-type enzyme |
-, 739701 |
| 1.2.7.11 | T349L |
the mutant shows reduced activity compared to the wild type enzyme |
-, 739701 |
| 1.2.7.11 | Y253A |
no enzyme activity at either 50 or 80°C |
719399 |
| 1.2.7.11 | Y253F |
kcat/Km for pyruvate is 3.9% of wild-type value, kcat/KM for 2-oxoglutarate is 16% of wild-type value |
-, 719399 |
| 1.2.7.11 | Y253W |
no enzyme activity at either 50 or 80°C |
719399 |
