EC Number   |
Protein Variants |
Reference |
|---|
   1.14.15.8 | A243S |
mutant engineered for steroid 9alpha- and 6beta-hydroxylation, 10fold increase in the selectivity towards 6alpha-hydroxylation of progesterone. Mutant produces 3.4% 15beta-hydroxy-progesterone plus 4.6% 11alpha-hydroxy-progesterone plus 2.4% 9alpha-hydroxy-progesterone plus 82.7% 6beta-hydroxy-progesterone |
746507 |
| 1.14.15.8 | F165L/A395E/G397V |
mutant engineered for steroid 9alpha- and 6beta-hydroxylation, 10fold increase in the selectivity towards progesterone 9lpha-hydroxylation. Mutant produces 11.3% 15beta-hydroxy-progesterone plus 17.2% 11alpha-hydroxy-progesterone plus 59.7% 9alpha-hydroxy-progesterone plus 6.8% 6beta-hydroxy-progesterone |
746507 |
| 1.14.15.8 | more |
recombinant reconstitution of the whole cell conversion of 11-keto-beta-boswellic acid in Bacillus megaterium strain MS941 by coexpression of heterologous redox chain of the P450, bovine adrenodoxin reductase, and bovine adrenodoxin, as well as a a NADPH-regenerating system |
-, 724061 |
| 1.14.15.8 | T248V |
specificity for 15beta-hydroxylation is improved |
699068 |
| 1.14.15.8 | G397P |
the mutant exhibits 2% of the wild-type activity, Vmax/Km for progesterone is 51.7fold lower than wild-type value |
697323 |
| 1.14.15.8 | E90V/D185G |
Vmax/Km for progesterone is 1.3fold higher than wild-type value |
697323 |
| 1.14.15.8 | K27R/I71T/I215T |
Vmax/Km for progesterone is 2.5fold higher than wild-type value |
697323 |
| 1.14.15.8 | I86T |
Vmax/Km for progesterone is 2fold higher than wild-type value |
697323 |
| 1.14.15.8 | A395L |
Vmax/Km for progesterone is 2fold lower than wild-type value |
697323 |
| 1.14.15.8 | Q398S |
Vmax/Km for progesterone is 5fold lower than wild-type value |
697323 |
