EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.87 | R87T |
site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate |
660918 |
| 1.1.1.87 | R87V |
site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate |
660918 |
| 1.1.1.87 | S80A |
the mutant shows decreased catalytic efficiency with homoisocitrate, isocitrate and 3-isopropylmalate compared to the wild type enzyme |
-, 760522 |
| 1.1.1.87 | T71V |
the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate, as well as increased catalytic efficiency with 3-isopropylmalate compared to the wild type enzyme |
-, 760522 |
| 1.1.1.87 | V135M |
site-directed mutagenesis, tetramer-to-dimer structural transition enhances the activity with isocitrate 1.6fold |
669109 |
| 1.1.1.87 | Y150F |
site-directed mutagenesis, inactive mutant |
739958 |
| 1.1.1.87 | Y150F |
site-directed mutagenesis, the active site mutant shows about 680fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly |
696350 |
