EC Number   |
Protein Variants |
Reference  |
|---|
    1.1.1.248 | S180A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
689571 |
| 1.1.1.248 | V106A |
site-directed mutagenesis, the mutant shows about 2fold increased activity compared to the wild-type enzyme |
689571 |
| 1.1.1.248 | Y236F |
site-directed mutagenesis, inactive mutant |
689571 |
| 1.1.1.248 | D107A |
increase in Km, 2fold decrease in kcat |
698937 |
| 1.1.1.248 | F104A |
increase in kcat |
698937 |
| 1.1.1.248 | F104A/I275A |
shows no substrate inhibition accompanied by a weak affinity for productive salutaridine binding, kcat is almost 2fold higher compared with the wild-type |
698937 |
| 1.1.1.248 | I275A |
increase in Km, shows an increase of velocity by a factor of 2.3 |
698937 |
| 1.1.1.248 | K186V |
moderate effects on substrate affinity, exhibits a turnover number similar to that of the wild-type |
698937 |
| 1.1.1.248 | L185A |
increase in Km, decrease in kcat |
698937 |
| 1.1.1.248 | L266A |
decrease in kcat |
698937 |
