EC Number   |
Reference |
|---|
    6.3.4.15 | apoenzyme, in complex with biotin, and complexed with biotinyl-5'-AMP, hanging drop vapor diffusion method, using |
728764 |
| 6.3.4.15 | EcBPL domains determination through X-ray crystallography at 2.3 A resolution, and determination of the crystal structure of EcBPL in complex with biotin |
691104 |
| 6.3.4.15 | hanging drop vapor diffusion method, using 8-12% (w/v) PEG8000 in 0.1 M Tris pH 7.5 or 8.0 and 10% (v/v) glycerol |
727901 |
| 6.3.4.15 | molecular docking of benzoxazolone derivatives in the active site region. Presence of a functional group attached either to the benzene ring or to the pyrole ring incresases binding efficacy |
745122 |
| 6.3.4.15 | oil-microbatch method using PEG 20000 as a precipitant |
671127 |
| 6.3.4.15 | PhBPL in complex with biotin, ATP and biotinyl-5'-AMP, X-ray diffraction and structure analysis at 1.6 A, 1.6 A, 1.45 A resolution, respectively. Structure-function relationship, modelling, overview |
691104 |
| 6.3.4.15 | purified recombinant enzyme, 0.003 ml of 5 mg/ml protein in 2.5 mM desthiobiotin in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM PMSF and 1 mM DTT, is mixed with 0.003 ml of reservoir solution containing 12-16% w/v of both PEG 4000 and PEG 8000 in 0.1 M HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 2.8 A resolution |
690261 |
| 6.3.4.15 | purified recombinant enzyme, complexed with biotinyl-5'-AMP, biotin, adenosine, or mutant biotin carboxyl carrier protein PhBCCPN76, X-ray diffraction structure determination at 2.0-2.7 A resolution, analysis, and modelling. The non-hydrolyzable intermediate analogue biotinol-5'-AMP does not provide analyzable complex crystals |
693053 |
| 6.3.4.15 | purified recombinant enzyme, hanging-drop vapour-diffusion method, 0.001 ml of 15 mg/ml protein solution is mixed with 0.001 ml of reservoir solution containing 8% PEG 8000 and 0.1 M TrisHCl pH 8.0., at 4°C or 20°C, crystals appear within 24 h, X-ray diffraction structure determination and analysis at 2.3 A resolution |
690291 |
| 6.3.4.15 | purified recombinant native and selenomethionine-labeled enzyme comprising residues 1-125, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution |
666059 |
