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EC Number
Crystallization (Commentary)
4.1.3.30
crystals of the inactive mutant C123S MICL in complex with isocitrate are obtained at room temperature by vapor diffusion in hanging drops. A crystal of C123S MICL in complex with pyruvate and succinate was obtained by overnight incubation of a C123S MICL/isocitrate complex crystal in a solution containing 35% PEG3350, 100 mM pyruvate and 100 mM succinate. This crystal also belongs to space group C2
4.1.3.30
native enzyme and bound with pyruvate/Mg2+, sitting and hanging drop vapor diffusion method
4.1.3.30
sitting drop vapor diffusion method
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