    3.7.1.1 | DMML in complex with Mg2+ and in complex with Mg2+ and 3,3-difluorooxalacetate, hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane (pH 7.5), and 20k polyethylene glycol 3350, or using 14k polyethylene glycol 6000 and 0.1 M MES (pH 6.5) |
693684 |
| 3.7.1.1 | OAH alone, in complex with a inhibitor 3,3-difluorooxalacetate, and in complex with reaction product oxalate, soaking of ligand-free OAH crystals in solution containing 0.2 M MnCl2 or 0.2 M MgCl2, 10 mM CaCl2, 2 mM 3,3-difluorooxalacetate, 0.1 M Na-HEPES, pH 7.5, and 30% v/v PEG 400 for 5-10 min yielded the desired enzyme inhibitor complex, X-ray diffraction structure determination and analysis at resolution limit of 1.30, 1.55, and 1.65 A , respectively, molecular replacement |
719879 |
