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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.1-
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.1crystal structure of mutant K180P-Galphai1 bound to a GTP analog
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.1Galphao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036, method screening, hanging-drop vapour-diffusion method, mixing of 0.0015 ml of 25 mg/ml protein solution containing 20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 150 mM NaCl, 2 mM DTT, with 0.0015 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES sodium salt, pH 7.5, and equilibration against 0.3 ml of reservoir solution, 8-10 days, 22°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, model fitting and refinement
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.1RGS4 complexed with Gialpha1-Mg2+-GDP-AlF4
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