3.6.1.55 | apoenzyme and its complexes with products (dCMP, CMP and 5-methyl-dCMP) or substrates (dCTP, CTP and 5-methyl-dCTP), using either 0.1 M imidazole pH 6.5, 1.0 M sodium acetate trihydrate (product complexes), 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 30% (w/v) PEG 4000 (substrate complexes), 0.1 M MES monohydrate pH 6.0, 20% (v/v) Jeffamine M-600 pH 7.0 or 0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30% (w/v) PEG 8000 (apoenzyme) |