3.5.1.42 | purified recombinant detagged enzyme free or in complex with nicotinate mononucleotide, or AMP/Mg2+, or ATP/Mg2+, or ribose-ADP/Mg2+, sitting drop vapor diffusion, mixing of 200 nl of protein with 200 nl of well solution containing 0.2 M Na2SO4, 0.1 M Bis-Tris propane, pH 6.5, and 20% w/v, PEG 3350 at 20°C, rodlike crystals, 5-7 days, X-ray diffraction structure determination and analysis at 1.98-2.46 A resolution, molecular replacement and modeling |
734285 |