EC Number   |
Reference |
|---|
   3.2.1.18 | (NH4)2SO4 at 0.65 to 0.7% saturation |
171154 |
| 3.2.1.18 | 56500 Da domain that retains full enzymatic activity, in presence of inhibitor 2-deoxy-2,3-dehydro-N-acetyl neuraminic acid. 2.5 A resolution, space group P212121 |
695355 |
| 3.2.1.18 | best crystals grow by hanging-drop vapor diffusion by equilibrating a 1 ml drop of protein in buffer (10 mM HEPES pH 7.2, 50 mM ammonium sulfate) with 1 ml reservoir solution containing 2.0-2.5 M ammonium sulfate and suspended over 1 ml reservoir solution. Structure determined at 2.2 A resolution |
677317 |
| 3.2.1.18 | carbohydrate-binding module CBM40 of sialidase, showing high affinity for sialic acid and specificity to alpha(2,3), alpha(2,6), and alpha(2,8)-linked sialosides |
698986 |
| 3.2.1.18 | catalytic domain of isoform NanI, to 0.97 A resolution, and complexes with its substrate sialic acid to 0.97A resolution, with transition-state analogue 2-deoxy-2,3-dehydro-N-acetylneuraminic acid to 1.5 A resolution, and with a covalent intermediate formed using a fluorinated sialic acid analogue to 1.2 A resolution |
698874 |
| 3.2.1.18 | comparison of the catalytic cleft plasticity of free and ligand-bound forms of Trypanosoma rangeli sialidase and Trypanosoma cruzi trans-sialidase using molecular dynamics simulations. The Trypanosoma cruzi enzyme has a very flexible, widely open catalytic cleft, mostly due to resiude W312 loop motion, in apo form. In ligan-bound form, the flexibility and solvent exposure is significantly reduced. The Trypanosoma rangeli sialidase maintains a more open catalytic cleft in both apo and holo forms |
696324 |
| 3.2.1.18 | crystallization of the catalytic domain by sitting-drop vapour-diffusion. Crystals belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 96.98, b = 69.41, c = 72.69 A and one monomer per asymmetric unit. The crystals diffract to at least 0.92 A |
663487 |
| 3.2.1.18 | crystallization of the enzyme alone or in complex with sialic acid, modeling |
654395 |
| 3.2.1.18 | free enzyme and in complex with N-acetylneuraminic acid or 2,3-dehydro-2-deoxy-N-acetylneuraminic acid, sitting drop vapor diffusion method, using 100 mM HEPES (pH 7.0) and 30% Jeffamine ED-2001 (pH 7.0), at 21°C |
702033 |
| 3.2.1.18 | free enzyme, sitting drop vapor diffusion method, using 100 mM HEPES (pH 7.0), 5% tacsimate, 7% (w/v) PEG 5000MME, at 21°C |
702033 |
