3.1.3.37 | purified enzyme with bound sedoheptulose-7-phosphate in the absence of AMP, hanging drop vapour diffusion method, mixing of 0.001 ml of 15 mg/ml protein in 50 mM Tris-HCl pH 7.9, 50 mM NaCl, 1 mM Mg-sedoheptulose-7-phosphate, and 0.2 M MgCl2, with 0.001 ml of reservoir solution containing 0.06 M Na HEPES pH 7.5, 0.12 M MgCl2, and 27% v/v PEG 400, and equilibration over 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.34 A resolution |
749507 |