EC Number   |
|---|
   3.1.1.81 | analysis of crystal structures of the enzyme with bound inhibitor N-homoserine lactone, and of the active site and substrate bound enzyme, modeling, overview |
| 3.1.1.81 | crystal structure of AidH at 1.29 A resolution and the structures of AidH-C4-HS (N-butanoyl homoserine), mutant S102G AidH-C6-HSL (N-hexanoyl homoserine lactone) and mutant E219G AidH-C6-HS (N-hexanoyl homoserine) complexes at resolutions of 1.09, 1.33 and 1.11 A, respectively, are determined |
| 3.1.1.81 | crystal structure of AidH at 1.29 A resolution and the structures of AidH-C4-HS (N-butanoyl-L-homoserine), mutant S102G AidH-C6-HSL (N-hexanoyl-L-homoserine lactone) and mutant E219G AidH-C6-HS (N-hexanoyl-L-homoserine) complexes at resolutions of 1.09, 1.33 and 1.11 A, respectively, are determined |
| 3.1.1.81 | crystal structure of AiiA F107W mutant determined in the presence and absence of N-decanoyl- L-homoserine is reported |
| 3.1.1.81 | crystal structure of the enzyme bound to phosphare, glycerol or N-hexanoyl-L-homoserine lactone. The crystal structure of AaL reveals a unique hydrophobic patch which can relate to its high specificity, and highlights a flexible active site loop which may be involved in substrate binding |
| 3.1.1.81 | D266N mutant, sitting drop vapor diffusion method, using 100 mM Tris, pH 7.5, 20% (w/v) PEG 4000, and 1.0 mM ZnCl2 |
| 3.1.1.81 | hanging drop vapor diffusion method, using 20-30% (w/v) PEG 8000 and 50 mM Tris-HCl buffer (pH 8.0) |
| 3.1.1.81 | in complex with phosphate and glycol |
| 3.1.1.81 | lactonase AiiA bound with two different concentrations of the ring-opened product of N-hexanoyl-L-homoserine lactone, and with the ring-opened product of the non-natural N-hexanoyl-L-homocysteine thiolactone, hanging drop method, 20 mg/mL protein in 20% glycerol, v/v, 80 mM Tris-HCl, 24% w/v PEG 4000, and 160 mM MgCl2, pH 8.5, X-ray diffraction structure determination and analysis at 0.95 and 1.4 A resolution and at 1.3 A resolution, respectively, modelling |
| 3.1.1.81 | native and selenomethionine-labeled enzyme, free or in complex with L-homoserine lactone, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution, structure modeling |
