EC Number   |
|---|
    3.1.1.3 | - |
| 3.1.1.3 | crystallization at a concentration of 26.0 mg/ml and 2.0 M sodium phosphate at pH 4.9 by the batch technique or with 10.0 mg/ml protein and 2.8 M phosphate at pH 7.8 using the hanging drop technique. Both crystallization conditions give isomorphous crystals during 2-4 weeks and room temperature |
| 3.1.1.3 | crystallized by vapor diffusion in sitting drops using polyethylene glycol and methyl-pentane-diol as precipitants in the presence of beta-octyl-glycopyranoside. Crystal structure of the enzyme is determined by isomorphous replacement and refined at 1.6 A resolution to an R-factor of 17.8% |
| 3.1.1.3 | crystallized in conditions from which the open, active conformation of the enzyme is expected. Its three-dimensional structure is determined independently in three different laboratories and is compared with the closed conformations of the closely related lipases from Pseudomonas glumae and Chromobacterium viscosum |
| 3.1.1.3 | hanging drop method, crystallographic analysis of wild-type and S136A mutant enzyme, 1.8 A resolution data |
| 3.1.1.3 | hanging drop vapour diffusion method, at 18°C, in 0.2 M ammonium sulfate, 0.1 M bis-tris pH 5.5, using 25% (w/v) PEG 3350 as a precipitant |
| 3.1.1.3 | mutant enzymes D157A and D337A, hanging drop vapour diffusion method, with 10% (w/v) polyethylene glycol 20 K, 0.2 M calcium acetate, 5 mM zinc acetate and 20% (v/v) ethylene glycol |
| 3.1.1.3 | purified recombinant enzyme in open conformation through complexion by inhibitor Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-(4-nitrophenyl) octylphosphonate, sitting drop vapour diffusion method, room temperature, 5 mg/ml protein in10 mM Tris-HCl, pH 8.0, 1% beta-octylpyranoside, equilibration against precipitation solution containing 25% 2-methyl-2,4-pentanediol, 20 mM CaCl2, 100 mM citrate, pH 5.6, several months, X-ray structure determination and analysis at 2.54 A resolution, structure scheme and modelling |
| 3.1.1.3 | sitting drop vapor diffusion method, using ammonia sulfate as the precipitant at 25°C |
| 3.1.1.3 | sitting drop vapour diffusion method, mixture of 13% 2-methyl-2,4-pentanediol and 0.2 M ammonium acetate in 0.05 M sodium citrate pH 5.5-5.6, at 18°C |
