EC Number   |
|---|
   2.7.7.6 | - |
| 2.7.7.6 | a large fragment of subunit PA bound to a fragment of subunit PB1, hanging dropn vapour diffusion method, 20°C, mixing of 0.001 ml of protein solution, containing 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 100 mM sodium chloride, and 0.001 ml of reservoir solution consisting of 100 mM Tris-HCl, pH 7.5, and 2.4 M sodium formate, X-ray diffraction structure determination and analysis at 2.3 A resolution |
| 2.7.7.6 | ammonium sulfate precipitation |
| 2.7.7.6 | crystal structure anaysis |
| 2.7.7.6 | crystal structure determination |
| 2.7.7.6 | crystal structure determination, structure including the A' subunit jaw and clamp head domains and RpoG and Rpo13 subunits |
| 2.7.7.6 | crystal structure of the core enzyme at 3.3 A resolution |
| 2.7.7.6 | crystal structure of the core enzyme at about 3.3 A resolution |
| 2.7.7.6 | crystallization of RNA polymerase II elongation complex. The purified paused complex forms crystals capable of X-ray diffraction to 3,5 A resolution. The complex remains active in the crystal and, in the presence of nucleoside triphosphates, can efficiently extend the transcript in situ |
| 2.7.7.6 | crystallization of the enzyme with DNA fragments, crystal growth is optimized using a nanoseeding technique, of the various crystals screened, one diffracts to 4.3 A resolution |
