EC Number   |
|---|
    2.7.2.3 | - |
| 2.7.2.3 | crystal structure of unliganded PGK, solved by molecular replacement to 1.8 A resolution, crystals belong to the P212121 space group with one molecule per asymmetric unit |
| 2.7.2.3 | crystal structures of Plasmodium falciparum phosphoglycerate kinase (PfPGK) is determined in open conformation in two different crystal forms to 2.7 A and 3 A resolution, respectively. In both structures a sulfate ion is bound in the ATP binding site and a second sulfate ion is located at the regulatory basic patch in the second crystal form |
| 2.7.2.3 | crystallization as native enzyme within 8-12 months or in complex with 3-phosphoglycerate and ATP analogue inhibitor beta,gamma-imido-adenosine-5'-triphosphate within 2-3 days, X-ray diffraction structure determination and analysis |
| 2.7.2.3 | crystallization from ammonium sulfate precipitate within 10 min, recrystallization |
| 2.7.2.3 | crystallization from ammonium sulfate precipitation |
| 2.7.2.3 | crystallization from ammonium sulfate precipitation overnight with gradually raising of temperature from 4°C to room temperature, in presence of ATP |
| 2.7.2.3 | crystallization from ammonium sulfate precipitation with 37% w/v by hanging or sitting drop vapour diffusion method, protein solution: 4 mg/ml, PIPES buffer, pH 6.8, 10 mM MgCl2, 1 mM ATP, 10% (NH4)2SO4, 22°C, 7-14 days, high resolution X-ray diffraction structure determination and analysis, rotating camera system |
| 2.7.2.3 | crystallization from ammonium sulfate precipitation, pH 7.0, room temperature, a few days, X-ray structure determination, in presence of 1% 1,4-dioxane and 68% ammonium sulfate, and analysis |
| 2.7.2.3 | crystallization via precipitation with ammonium sulfate stepwise from 30-35% to 65% w/v within 24 h |
