EC Number   |
|---|
    2.6.1.42 | - |
| 2.6.1.42 | 7.6 mg/ml purified protein in 25 mM HEPES, pH 7.5, 25 mM NaCl, 20 mM DTT, 20 mM EDTA, 2.5% glycerol, hanging drop vapor diffusion method at 20°C with microseeding in a protein to microseed solution of drop ratio of 1:1, soaking of crystals in reservoir solution containing 10-20 mM DTT and inhibitors, X-ray diffraction structure determination and analysis |
| 2.6.1.42 | BCATm, orthorhombic space group P2(1)2(1)2(1), monoclinic P2(1), trigonal P3(2) |
| 2.6.1.42 | crystal structure analyses, PDB IDs 1KTA, 1KT8, and 2HG8 |
| 2.6.1.42 | crystallized from recombinant Escherichia coli |
| 2.6.1.42 | crystallized in 2 crystal systems, monoclinic by sitting drop crystallization and tetragonal by hanging drop, monoclinic space group C2, a=93.9, b=143.6, c=143.9 and beta=134.3°, tetragonal space group P422 or P4(1)22 and cell dimensions of a=b=101 A and c=249 A |
| 2.6.1.42 | cytosolic isoform in complex with gabapentin or 4-methylvalerate, mitochondrial isoform in complex with gabapentin |
| 2.6.1.42 | diffraction to 2.5 A resolution, space group P212121 |
| 2.6.1.42 | hanging drop method, ammonium sulfate, PEG 3350 |
| 2.6.1.42 | hanging drop vapor diffusion method, using 1% (w/v) tryptone, 50 mM HEPES pH 7.0, and 12% (w/v) polyethylene glycol 3350 |
