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EC Number Crystallization (Commentary)
Display the reaction diagram Show all sequences 2.4.1.250vapor diffusion under silicon oil. The structure of MshA is determined both in the absence of substrates and in a complex with UDP and 11D-myo-inositol 3-phosphate. The structure of unliganded CgMshA (APO form) is determined by single isomorphous replacement with anomalous scattering utilizing a CuKalpha home source and a mercury derivative to 2.1 A resolution. Crystallization of CgMshA in the presence of UDP or UDP-GlcNAc results in a tetragonal crystal form. The structure of MshA from Corynebacterium glutamicum is determined both in the absence of substrates and in a complex with UDP and 1L-myo-inositol-1-phosphate
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