2.1.1.35 | native and selenomethionylated purified enzyme PabTrmU54 in complex with S-adenosyl-L-homocysteine, hanging-drop by vapor diffusion method, 0.001 ml of a mixture of protein and S-adenosyl-L-methionine in a 1:2 molar ratiomixed with 0.001 ml of 0.6 ml reservoir solution containing 15% PEG 8000, 0.05 M ammonium sulfate, 0.1 M sodium citrate, pH 5.6,18°C, few days, X-ray diffraction structure determination and analysi at 1.9 A resolution, molecular replacement, modelling |