EC Number   |
Reference  |
|---|
   2.1.1.220 | sitting-drop vapor-diffusion method at 19°C. Crystal structure of TrmI, in complex with S-adenosyl-L-homocysteine, is determined at 1.7 A resolution. The conserved residues that form the catalytic cavity (D170, Y78, and Y194) are essential for fashioning an optimized shape of the catalytic pocket |
699528 |
| 2.1.1.220 | crystal structure of Rv2118c in complex with S-adenosyl-L-methionine has been determined at 1.98 A resolution |
712735 |
| 2.1.1.220 | purified recombinant His-tagged TRM6-TRM61 complex, from 0.1 M KCl, 0.1 M Tris-HCl, pH 8.0, 25% w/v PEG 2000 MME, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement |
718504 |
| 2.1.1.220 | ligand-free TrmI, X-ray diffraction structure determination and analysis at 1.65 A resolution |
719192 |
| 2.1.1.220 | TrmI complexed with S-adenosyl-L-methionine, X-ray diffraction structure determination and analysis at 1.98 A resolution |
719192 |
| 2.1.1.220 | TrmI complexed with S-adenosyl-L-methionine, X-ray diffraction structure determination and analysis at 2.2 A resolution |
719192 |
| 2.1.1.220 | TrmI protein complexed as tetramer with S-adenosyl-L-homocysteine or as monomer with S-adenosyl-L-methionine, X-ray diffraction structure determination and analysis at 2.05-2.6 A or 1.6 A resolution, respectively |
719192 |
| 2.1.1.220 | TrmI protein complexed with S-adenosyl-L-homocysteine, X-ray diffraction structure determination and analysis at 1.7 A resolution |
719192 |
| 2.1.1.220 | TrmI-61 protein complexed with S-adenosyl-L-methionine, X-ray diffraction structure determination and analysis at 2.5 A resolution |
719192 |
| 2.1.1.220 | purified enzyme mutant D170A and Y78A in complex with S-adenosyl-L-methionine, hanging drop vapor diffusion method, mixing of 10 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, 100 mM KCl, and 2mM S-adenosyl-L-methionine with reservoir solution containing 2.4 M ammonium sulfate and 10% v/v isopropanol for mutant D170A and 2.1 M ammonium sulfate and 8% v/v isopropanol for mutant Y78A, X-ray diffraction structure determination and analysis at 3.1 A and 2.6 A resolution, respectively. Crystallization assays of enzyme TrmI Y194A lead to poorly diffracting crystals |
735777 |
