EC Number   |
|---|
   1.8.4.16 | oxidized (ox) and reduced (red) periplasmic domains c-DsbD and n-DsbD, using 8-20% (w/v) PEG 6000, 100 mM MES (pH 6.4), and 20 mM ZnCl2 for n-DsbDox, 2.5 M ammonium sulfate and 0.1 M Tris (pH 9.1) for n-DsbDred, 5% (w/v) PEG 400 and 1.7-2.2 M citrate/citric acid (pH 7.0-7.8) for c-DsbDox, and 0.2 M zinc acetate dehydrate, 0.1 M sodium cacodylate trihydrate (pH 7.5), and 18% (w/v) PEG 8000 for c-DsbDred |
| 1.8.4.16 | periplasmic domains n-DsbD and c-DsbD in both redox states, hanging drop vapor diffusion method, using 18-20% (w/v) PEG 6000, 0.1 M MES pH 6.4, 0.02 M ZnCl2 (for the oxidized state of n-DsbD), 2.5 M ammonium sulfate, 0.1 M Tris pH 9.1 (for the reduced state of n-DsbD), 5% (w/v) PEG 400, 1.7-2.2 M citrate/citric acid pH 7.0-7.8 (for the oxidized state of c-DsbD), or 0.2 M zinc acetate dihydrate, 0.1 M sodium cacodylate trihydrate pH 7.5, 18% (w/v) PEG 8000 (for the reduced state of c-DsbD) |
| 1.8.4.16 | using 25% (w/v) poly(ethylene glycol) 4000, 0.1 M sodium acetate, pH 4.6, and 0.2 M NH4SO4 |
