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EC Number	Crystallization (Commentary)
1.17.7.4	crystallized under anaerobic conditions. Its structure is determined by the multiple anomalous dispersion method and refined to final R/Rfree factors of 17.5/19.5% at 2.2 A resolution. LytB is a momoner which is essentially composed of three related alpha/beta-domains arranged in a cloverleaf form with the Fe/S-cluster in its center
1.17.7.4	purified recombinant IspH, sitting drop vapour diffusion method at 20°C, 0.004 ml of 35 /ml protein in 50 mM Tris-HCl, pH 8.0, is mixed with 0.004 ml of reservoir solution containing 100 mM Bis-Tris-HCl, pH 6.5, 200 mM lithium sulfate and 25% PEG 3350, final pH is 6.8, crystal soaking in 2 M lithium sulfate, pH 6.5, before freezing, X-ray diffraction structure determination and analysis at 1.4 A resolution, molecular replacement, modelling

EC Number

Crystallization (Commentary)

1.17.7.4

crystallized under anaerobic conditions. Its structure is determined by the multiple anomalous dispersion method and refined to final R/Rfree factors of 17.5/19.5% at 2.2 A resolution. LytB is a momoner which is essentially composed of three related alpha/beta-domains arranged in a cloverleaf form with the Fe/S-cluster in its center

1.17.7.4

purified recombinant IspH, sitting drop vapour diffusion method at 20°C, 0.004 ml of 35 /ml protein in 50 mM Tris-HCl, pH 8.0, is mixed with 0.004 ml of reservoir solution containing 100 mM Bis-Tris-HCl, pH 6.5, 200 mM lithium sulfate and 25% PEG 3350, final pH is 6.8, crystal soaking in 2 M lithium sulfate, pH 6.5, before freezing, X-ray diffraction structure determination and analysis at 1.4 A resolution, molecular replacement, modelling

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Release 2023.1 (January 2023)