EC Number   |
|---|
    1.17.4.1 | alpha subunit, in apo form, in complex with AMP analogue 5'-adenylyl-beta,gamma-imidodiphosphate, with 5'-adenylyl-beta,gamma-imidodiphosphate and CDP, with 5'-adenylyl-beta,gamma-imidodiphosphate and UDP, with dGTP and ADP, TTP and GDP. Binding of specificity effector rearranges loop 2 and moves residue P294 out of the catalytic site, accomodating substrate binding. substrate binding further rearranges loop2. Cross-talk occurs largely through R293 and Q288 of loop 2. Substrate ribose binds with its 3 hydroxyl closer than its 2 hydroxyl to residue C218 of the catalytic redox pair |
| 1.17.4.1 | B2 subunit, hanging drop method, crystallization in 20% polyethylene glycol 4000, 200 mM NaCl, 0.3% dioxane, 50 mM ethyl mercuric thiosalicylate, 50 mM MES buffer, pH 6.0, orthorhombic crystals, crystal structure at 2.2 A resolution |
| 1.17.4.1 | B2 subunit, hanging drops of 0.01 ml containing 25 mg/ml of enzyme with 1 ml of 1.5 M ammonium sulfate in the well and 750 mM ammonium sulfate starting concentration in the drop, pH 6.0, crystals appear after 1 week at room temperatur |
| 1.17.4.1 | complex between NrdIox and MnII 2-NrdF, Two NrdI and two NrdF molecules are present in the asymmetric unit, X-ray diffraction structure determination and analysis at 2.5 A resolution |
| 1.17.4.1 | crystals are grown using the hanging drop vapor diffusion technique |
| 1.17.4.1 | free radical subunit R2, basic motif is a bundle of eight long helices. R2 dimer has two equivalent dinuclear iron centers. Iron atoms have both histidine and carboxyl acid ligands and are bridged by the carboxylate group of E115. The essential residue Y122 is buried inside the protein and the tyrosyl radical cannot participate directly in hydrogen abstraction |
| 1.17.4.1 | hanging drop method |
| 1.17.4.1 | hanging drop method, crystal structures of the dimeric class II RNR in complex with four cognate allosteric specificity effector-substrate pairs (dTTP-GDP, dGTP-ADP, dATP-CDP or dATP-UDP), as well as structures with only the different effectors (dATP, dTTP or dGTP) |
| 1.17.4.1 | low-resolution crystal structure of an alpha2beta2 complex |
| 1.17.4.1 | mutant Y122H of R2 protein subunit |
