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Results 1 - 8 of 8
EC Number
Crystallization
Reference
crystal structure of tetrameric cytidine deaminase at 2.0 A resolution, hanging drop vapour-diffusion method
hanging drop vapour diffusion method. R56A and R56Q crystallize in the same space group as the wild-type enzyme with two subunits in the asymmetric unit, whereas C53H/R56Q can not crystallize in this crystal form but is crystallized in another space group, P3(2)21, with a full tetramer in the asymmetric unit
in complex with tetrahydrouridine, 3-deazauridine, or cytidine. Two alternate conformations of R68 influence zinc-product interaction; purified recombinant CDA complexed with either tetrahydrouridine, 3-deazauridine, or cytidine, hanging drop vapor diffusion technique, 25°C, protein solution, containing 10-15 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 1 mM DTT, and 5 mM ligand, is mixed with crystallization solution containing 2.0 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, crystals appear after a few hours or several days, respectively, X-ray diffraction structure determination and analysis at 1.48-2.36 A resolutions, molecular replacement
in complex with inhibitor diazepinone riboside. Inhibitor is able to establish a canonical pi/pi interaction with key active site residue F137
comparison of theoretically predicited model and experimentally elucidated structure
hanging drop vapor diffusion method
purified CDA in complex with uridine and deoxyuridine, hanging drop vapor diffusion method, 0.002 ml of 12 mg/ml protein in 20 mM Tris-HCl pH 7.5 is mixed with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5 and 4.3 M sodium chloride, ligands uridine and deoxyuridine are added by soaking method, X-ray diffractiuon structure determination and analysis at 2.4 and 1.9 A resolution,molecular dynamics simulation, structure modeling
Results 1 - 8 of 8