EC Number   |
pH Minimum |
pH Maximum |
Reference |
|---|
   1.4.1.3 | 6.5 |
8 |
pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity |
744858 |
| 1.4.1.3 | 7 |
8 |
25-30% less active at pH 7.0 than at pH 7.5 and 8.0 |
656871 |
| 1.4.1.3 | 7 |
8 |
90-100% activity |
391621 |
| 1.4.1.3 | 7 |
8 |
equally active at pH 7.0 and 8.0 |
656871 |
| 1.4.1.3 | 7 |
8 |
lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination |
725866 |
| 1.4.1.3 | 7 |
9 |
pH 7.0: about 50% of maximal activity, pH 9.0: about 35% of maximal activity |
744858 |
| 1.4.1.3 | 7.4 |
8.5 |
- |
671289 |
| 1.4.1.3 | 7.5 |
8 |
half activity of glutamate deamination compared to pH 9.5 |
391634 |
| 1.4.1.3 | 7.5 |
8.8 |
NAD(P)H + 2-oxoglutarate + NH3, more than 50% of activity maximum at pH 7.5 and 8.8 |
391608 |
| 1.4.1.3 | 7.75 |
8 |
maximal specific activity in TRA buffer |
700428 |
