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EC Number	pH Optimum Minimum	pH Optimum Maximum	Commentary	Reference
2.5.1.65	6.7	-	-	637385
2.5.1.65	6.7	-	assay at	737868
2.5.1.65	7	-	assay at	738534
2.5.1.65	7.3	8.1	at 85°C	655576
2.5.1.65	7.3	8.1	dependent on the substrate, overview	655576
2.5.1.65	7.5	-	assay at	738193
2.5.1.65	9	-	the pH optimum for the dephosphorylation reaction is around pH 9.0, most likely because the spontaneous hydrolysis of the aminoacrylate intermediate is faster at this pH value, resulting in an increased turnover rate in the absence of the sulfur donor	738534

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Release 2023.1 (January 2023)