EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.3.1 | -999 |
- |
more |
- |
651016, 651458, 652838, 665760, 94573, 94630 |
3.1.3.1 | -999 |
- |
more |
kcat/KM (1/Msec) wild-type: 140000, mutant R166K: 20, mutant R166S: 24 |
690980 |
3.1.3.1 | -999 |
- |
more |
wild-type enzyme: kcat/KM (1/M*sec) (4-nitrophenyl phosphate): 33000000, (3-nitrobenzyl phosphate): 18000000, (methyl phosphate): 1200000, (methyl 4-nitrophenyl phosphate): 18, (bis-4-nitrophenyl phosphate): 0.05, (4-nitrophenyl sulfate): 0.01 |
693669 |
3.1.3.1 | 0.000029 |
- |
4-nitrophenyl phosphate |
mutant enzyme D101A/R166S/D153A/E322Y/K328A, at pH 8.0 and 25°C |
750399 |
3.1.3.1 | 0.000057 |
- |
4-nitrophenyl phosphate |
mutant enzyme R166S/E322Y/K328A, at pH 8.0 and 25°C |
750399 |
3.1.3.1 | 0.00021 |
- |
4-nitrophenyl phosphate |
pH 8.0, 25°C, recombinant mutant T59R |
652408 |
3.1.3.1 | 0.00024 |
- |
4-nitrophenyl phosphate |
mutant enzyme D101A/D153A/E322Y/K328A, at pH 8.0 and 25°C |
750399 |
3.1.3.1 | 0.00032 |
- |
4-nitrophenyl phosphate |
mutant enzyme D101A/R166S/D153A/E322Y, at pH 8.0 and 25°C |
750399 |
3.1.3.1 | 0.00073 |
- |
4-nitrophenyl phosphate |
mutant enzyme D153A/E322Y/K328A, at pH 8.0 and 25°C |
750399 |
3.1.3.1 | 0.00089 |
- |
4-nitrophenyl phosphate |
mutant enzyme D101A/E322Y/K328A, at pH 8.0 and 25°C |
750399 |