EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
5.4.99.5 | 257900 |
- |
chorismate |
37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine |
650528 |
5.4.99.5 | 258000 |
- |
chorismate |
37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine |
650528 |
5.4.99.5 | 365600 |
- |
chorismate |
37°C, pH 7.8, DELTA102-285 |
650528 |
5.4.99.5 | 366000 |
- |
chorismate |
37°C, pH 7.8, DELTA102-285 |
650528 |
5.4.99.5 | -999 |
- |
more |
CM spezific activity in Escherichia coli extracts: Control (M. Tuberculosis) Sp act (U/mg) 0.889 Cloned extract/control 1 AroQMt (Rv0948c) Sp act (U/mg) 127.38 Cloned extract/control 143.28 *AroQMt (Rv1885c) Sp act (U/mg) 94.23 Cloned extract/control 105.9. CM specific activity in Escherichia coli cells expressing either the AroQMt or the *AroQMt protein is determined to be, repectively, 143- and 106-fold higher than the enzyme activity obtained for Escherichia coli cells carrying the pET-23a(+) expression vector |
674346 |
5.4.99.5 | -999 |
- |
more |
R90Cit 10E4-fold decrease in the catalytic activity of kcat. R90K 10E4-fold decrease in the catalytic activity of kcat/Km is obtained |
675611 |
5.4.99.5 | -999 |
- |
more |
The kcat of the active complementations (position 7, 32, 35, 48, 81 and 85) is shown |
672268 |
5.4.99.5 | -999 |
- |
more |
turnover of mutant H189N enzyme is lower than 0.0025 per second |
390552 |
5.4.99.5 | 94100 |
- |
prephenate |
37°C, pH 7.8, in presence of P-protein |
650528 |
5.4.99.5 | 94140 |
- |
prephenate |
37°C, pH 7.8, in presence of P-protein |
650528 |