EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.2.1.18 | 0.82 |
- |
phenyl alpha-sialoside |
pH 5.25, 37°C, mutant enzyme Y370G |
678161 |
3.2.1.18 | 0.88 |
- |
4-nitrophenyl alpha-D-N-acetylneuraminic acid |
- |
703911 |
3.2.1.18 | 0.9 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
wild type enzyme, at 37°C, pH not specified in the publication |
752324 |
3.2.1.18 | 1.57 |
- |
4-nitrophenyl alpha-sialoside |
wild-type, hydrolysis, pH 7.6, 25°C |
696254 |
3.2.1.18 | 2.9 |
- |
(trifluoromethyl)umbelliferyl alpha-sialoside |
wild-type, hydrolysis, pH 7.6, 25°C |
696254 |
3.2.1.18 | 8 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant mutant M87G, pH 5.0, 37°C |
717641 |
3.2.1.18 | 13.3 |
- |
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid |
isoform Am2085, at pH 6.5 and 37°C |
750235 |
3.2.1.18 | 13.3 |
- |
phenyl beta-sialoside |
pH 5.25, 37°C, mutant enzyme Y370G |
678161 |
3.2.1.18 | 23 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant wild-type enzyme, pH 5.0, 37°C |
717641 |
3.2.1.18 | 27 |
- |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid |
recombinant mutants A160G and H277F, pH 5.0, 37°C |
717641 |