EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.87 | 0.4 |
- |
NAD+ |
native enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 0.5 |
- |
3-vinylmalate |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.59 |
- |
trisodium (2S,3R)-2-(carboxylatomethoxy)-3-hydroxybutanedioate |
- |
685539 |
1.1.1.87 | 0.61 |
- |
ethylmalate |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.7 |
- |
(2R,3S)-3-propylmalic acid |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.71 |
- |
isocitrate |
mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.77 |
- |
3-vinylmalate |
pH 7.8, 28°C, recombinant enzyme |
667934 |
1.1.1.87 | 0.78 |
- |
(2R,3S)-3-(2-hydroxyethyl)malate |
pH 7.8, 36°C, recombinant enzyme |
667934 |
1.1.1.87 | 1 |
- |
isocitrate |
mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 1.1 |
- |
isocitrate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |