EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
5.3.99.2 | -999 |
- |
thermal unfolding is completely reversible at pH 4.0, with the presence of an intermediate state I between the native state N and the unfolded state U. Transition temperatures of the N-I and I-U transitions are 48.2 and 60.3°C, respectively. In the intermediate state, the main chain retains its characteristic beta-sheet structure without side.chain interactions |
692292 |
5.3.99.2 | 57.4 |
- |
melting temperature, mutant C89A/C186A |
691128 |
5.3.99.2 | 59.6 |
- |
melting temperature, mutant C186A |
691128 |
5.3.99.2 | 60 |
- |
purified recombinant enzyme, sodium phosphate, pH 7.0, containing 5% v/v ethanol, stable up to |
726907 |
5.3.99.2 | 65 |
90 |
purified recombinant enzyme, thermal unfolding in the absence and presence of small lipophilic ligands, two-state unfolding transition, overview |
726907 |
5.3.99.2 | 69.3 |
- |
melting temperature, wild-type |
691128 |
5.3.99.2 | 69.4 |
- |
melting temperature, mutant C65A |
691128 |