EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.1.1.42 | -999 |
- |
the enzyme is incubated in Tris/EDTA/Mg2+ buffer containing either 0.5 M or 3 M KCl. The thermal stability of the enzyme is substantially reduced at the lower KCl concentration, with concomitant differences in the activation energies for the thermal inactivation process. Omission of Mg2+ from the incubation buffer greatly accelerates the rate of loss of activity, and its presence plus that of 3 M KCl are recommended for storage of the enzyme |
719515 |
| 1.1.1.42 | -999 |
- |
thermal denaturation is an irreversible process |
727835 |
| 1.1.1.42 | -999 |
- |
thermal stability of wild-type and chimeric enzymes |
722205 |
| 1.1.1.42 | 10 |
15 |
stable at, purified recombinant His-tagged isozyme IDH-IIa |
740185 |
| 1.1.1.42 | 10 |
15 |
stable at, purified recombinant His-tagged isozyme IDH-IIb |
740185 |
| 1.1.1.42 | 15 |
- |
isoenzyme II |
286791 |
| 1.1.1.42 | 20 |
- |
isoenzyme II |
286787 |
| 1.1.1.42 | 30 |
- |
50% loss of activity in 1 h |
286779 |
| 1.1.1.42 | 30 |
- |
purified recombinant enzyme, 20 min, stable |
723152 |
| 1.1.1.42 | 30 |
- |
the enzyme loses 74% of the activity after incubation for 10 min at 30°C |
761052 |
