EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.4.1.13 | -20 |
- |
complete loss of activity after 10 days |
391455 |
1.4.1.13 | -10 |
- |
stable for at least 6 weeks |
391452 |
1.4.1.13 | 0 |
- |
stable for 6-8 h in crude extract |
391458 |
1.4.1.13 | 4 |
- |
unprotected enzyme loses 90% of activity in 48 hours, protected enzyme, addition of 5 mM EDTA and 5 mM 2-mercaptoethanol or 1 mM DTT retains 80 to 90% of the activity after 48 hours |
391455 |
1.4.1.13 | 40 |
- |
10 min, 80% loss of activity, enzyme in crude extract |
391458 |
1.4.1.13 | 45 |
- |
3 min, 80% loss of activity, enzyme in crude extract |
391458 |
1.4.1.13 | 45 |
- |
the enzyme retains its full activity after heating for 10 min in 0.1 M potassium phosphate buffer, pH 6.8 |
391448 |
1.4.1.13 | 55 |
- |
10 min, in 0.1 M potassium phosphate buffer, pH 6.8, 70-80% loss of activity |
391448 |
1.4.1.13 | 70 |
100 |
purified enzyme, 10 min, stable up to 90°C |
725886 |
1.4.1.13 | 80 |
- |
10 min, 35% loss of activity |
391451 |