EC Number   |
|---|
   7.2.1.3 | cloning and recombinant expression of untagged four CYB561 isoforms in yeast YPH499 cells, recombinant expression of two C-terminally His10- or Strep-II-tagged CYB561 paralogues in either Escherichia coli or in Pichia pastoris. Functional expression of the enzyme in Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 deficient in ferric reductase activity |
| 7.2.1.3 | Dcytb-EGFP is expressed in tetracycline-off Madin-Darby canine kidney cells |
| 7.2.1.3 | expressed as a His-tagged fusion protein in Escherichia coli |
| 7.2.1.3 | expressed in a Saccharomyces cerevisiae DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity |
| 7.2.1.3 | expressed in a Saccharomyces cerevisiae strain S288C DELTAfre1DELTAfre2 mutant, which lacks almost all of its plasma membrane ferrireductase activity |
| 7.2.1.3 | expressed in Escherichia coli |
| 7.2.1.3 | expressed in Saccharomyces cerevisiae, strain YPH499 |
| 7.2.1.3 | expressed in the Saccharomyces cerevisiae mutant strain S288C DELTAfre1DELTAfre2 that lacks plasma membrane ferrireductase activity |
| 7.2.1.3 | expressed in Xenopus laevis oocytes |
| 7.2.1.3 | expression in Escherichia coli |
