EC Number   |
|---|
    6.4.1.1 | - |
| 6.4.1.1 | a Pseudomonas aeruginosa strain carrying the T7 polymerase gene can serve as a host for the overexpression of Mycobacterium smegmatis alpha4 under the control of the T7 promoter from a broad-host-range conjugative plasmid. Overexpression occurrs both in aerobic (LB medium) and nitrate-respiring anaerobic (LB medium plus glucose and nitrate) cultures. The latter system presents a simpler option because it involved room temperature cultures in stationary screw-cap bottles. Developed of a Pseudomonas aeruginosa DELTApyc strain that allows the expression of recombinant PYCs in the absence of the native enzyme |
| 6.4.1.1 | a Pseudomonas aeruginosa strain carrying the T7 polymerase gene can serve as a host for the overexpression of Pseudomonas aeruginosa alpha4beta4 PYC under the control of the T7 promoter from a broad-host-range conjugative plasmid. Overexpression occurs both in aerobic (LB medium) and nitrate-respiring anaerobic (LB medium plus glucose and nitrate) cultures. The latter system presents a simpler option because it involved room temperature cultures in stationary screw-cap bottles. Development of a Pseudomonas aeruginosa DELTApyc strain that allows the expression of recombinant PYCs in the absence of the native enzyme |
| 6.4.1.1 | codon-optimized expression in CHO cells |
| 6.4.1.1 | coexpressed with human erythropoietin in BHK-21 cells |
| 6.4.1.1 | coexpression of pyruvate carboxylase 1 isozyme (Pyc1) with an N-terminal myc tag, together with constructs encoding either the biotin carboxylase domain or the transcarboxylase-biotin carboxyl carrier domain, each with an N-terminal 9-histidine tag |
| 6.4.1.1 | DNA and amino acid sequence determination and analysis |
| 6.4.1.1 | DNA and amino acid sequence determination and analysis, expression mutant enzymes and of the isolated biotin carboxylase domain |
| 6.4.1.1 | DNA and amino acid sequence determination and analysis, genetic structure, key cognate transcription factors regulating tissue-specific expression, transcriptional regulation, overview |
| 6.4.1.1 | DNA and amino acid sequence determination and analysis, genetic structure, key cognate transcription factors regulating tissue-specific expression. Five species of enzyme mRNAs have been reported, each having the same coding sequence but differing in their 5'-untranslated regions. These mRNA variants are the product of alternative splicing of two primary transcripts initiated from two alternative promoters, the proximal and the distal promoters. Neither of these promoters contains a TATA box but both possess multiple GC boxes. Production of specific forms of PC mRNA are linked to certain physiological states, i.e. development, gluconeogenesis and lipogenesis. Two pancreatic isletspecific transcription factors, i.e. pancreatic duodenal homeobox-1or PDX1, and v-MAFA, are involved in transcriptional regulation of the enzyme in INS1 cells. Identification of a putative cAMP-responsive element in the proximal promoter of the rat PC gene, transcriptional regulation, overview |
