EC Number |
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6.3.1.13 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme |
6.3.1.13 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as N-terminally His-tagged enzyme |
6.3.1.13 | expression of His-tagged enzyme in Escherichia coli strain BL21 |
6.3.1.13 | gene mshC, construction of three N-terminal-MshC fusion proteins where N-terminal tags include the B1 domain of 1. streptococcal protein G to give GB1-MshC, 2. glutathione-S-transferase to give GST-MshC, and 3. maltose binding protein to give MBP-MshC, for expression in M. smegmatis, expression in enzyme-deficient mutant strain mc2155, i.e. I64 L205P, optimization of recombinant enzyme expression, overview |
6.3.1.13 | gene mshC, enzyme expression analysis |
6.3.1.13 | gene mshC, expression in the mshC-deficient mutant strain I64 of Mycobacterium smegmatis, expression as insoluble protein in Escherichia coli |