EC Number   |
|---|
    5.4.99.1 | - |
| 5.4.99.1 | 13C,15N-labeled MutS, the coenzyme B12-binding subunit of glutamate mutase prepared by overexpression from Escherichia coli |
| 5.4.99.1 | expressed in Escherichia coli |
| 5.4.99.1 | expression in Escherichia coli |
| 5.4.99.1 | genes mutS and mutE, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) pLysS |
| 5.4.99.1 | glutamate mutase S component MutS. Induction strategy to enhance the level of protein expression in bacteriophage T7 expression system in Escherichia coli. Yield of purified protein is increased threefold by reducing the induction temperature to 20°C and using 0.2% lactose and 50 mg per l IPTG simultaneously as inducers |
| 5.4.99.1 | individual expression of SanU and SanV in Escherichia coli |
| 5.4.99.1 | overexpression of component E and S in Escherichia coli |
| 5.4.99.1 | overexpression of polypeptide chains sigma and epsilon in Escherichia coli |
| 5.4.99.1 | polypeptide chains sigma and epsilon expressed in Escherichia coli |
