EC Number   |
|---|
   5.1.3.B12 | DNA and amino acid sequence determination and analysis of the D-psicose 3-epimerase gene, recombinant expression in Escherichia coli strain BL21(DE3), co-expression with xylose isomerase gene from Escherichia coli strain MG1665 from plasmid pET-28a-DPE-xylA |
| 5.1.3.B12 | expression in Escherichia coli. It has no significant sequence similarities to D-tagatose 3-epimerase. It is genetically different from other sugar epimerases |
| 5.1.3.B12 | gene agtu, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 5.1.3.B12 | gene agtu, subcloning in Escherichia coli strain ER2566, expression of recombinant His-tagged enzyme mutant I33L-S213C in Agrobacterium tumefaciens at high level, optimal at optimal at 60x02C, pH 8.5, 4 g/l cells, and 700 g/l D-fructose. The recombinant cells produced 230 g/l D-psicose after 40 min, with a conversion yield of 33% w/w, a volumetric productivity of 345 g/l/h, and a specific productivity of 86.2 g/g/h |
| 5.1.3.B12 | gene dpe, DNA and amino acid sequence determination and analysis, recombinant expression of the engineered chimeric Smt3-D-psicose 3-epimerase conjugate in Escherichia coli strain BL21 |
| 5.1.3.B12 | gene dpe, functional recombinant expression of the enzyme in thermotolerant Kluyveromyces marxianus strain CICC1911, pRS42H-dpe is transformed into the thermotolerant cells of strain CICC1911 by using the LiAc/salmon sperm carrier DNA/PEG method |
| 5.1.3.B12 | the gene AAL45544.1 from Agrobacterium tumefaciens str. C58 is modified by artificial synthesis for overexpression in Escherichial coli, expression in Escherichia coli |
