EC Number   |
|---|
    5.1.1.1 | - |
| 5.1.1.1 | 31-54% sequence homologies with Bacillus subtilis and Salmonella typhimurium dadB and alr enzymes |
| 5.1.1.1 | amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products |
| 5.1.1.1 | amplified by PCR, cloned and overexpressed with pOPINB in Escherichia coli Rosetta pLysS cells, His-tagged product |
| 5.1.1.1 | amplified from genomic DNA from Enterococcus faecalis via PCR, integrated into pET22b, transformation of Escherichia coli BL21 (DE3) for overexpression as His-tagged protein |
| 5.1.1.1 | analyzation of alanine racemase-transcripts (RT-PCR) during growth and sporulation of wild-type shows low transcription level during early and higher during late sporulation states, analyzed expression level (immunoblotting) shows only alanine racemase during late sporulating states, growth rate of alr-gene knockout mutant and wild-type are identical, mutant only produces half as many spores as wild-type, mutant and wild-type show same resistance against heat, lysozyme and organic solvents, germination takes place in mutant at lower levels of L-alanine (a suggested germination activator) than in wild-type (D-alanine ist suggested to inhibit germination when unfavourable growth conditions), conversion of phase-bright spores to phase-dark germinating cells takes already place within mother cells of the alr mutant (phase dark to phase light), not in wild-type cells resulting in non-resistant mutant-germinants and resistant wild-type-spores respectively |
| 5.1.1.1 | cloning of two independent alanine racemases in Escherichia coli: Alr and DadX |
| 5.1.1.1 | dadB |
| 5.1.1.1 | enzyme expression in Escherichia coli strain BL21(DE3)/pETALR |
| 5.1.1.1 | enzyme expression in Escherichia coli strain BL21(DE3)/pMB1978 |
