EC Number   |
|---|
   4.6.1.2 | - |
| 4.6.1.2 | beta1 (residues 1-194) and beta2 (residues 1-217) homodimers expressed in Escherichia coli |
| 4.6.1.2 | by baculovirus expression system |
| 4.6.1.2 | catalytic domains (alphacat and betacat) of alpha1beta1 soluble guanylate cyclase are expressed in Escherichia coli |
| 4.6.1.2 | cloning of isozyme GC-A, DNA and amino acid sequence determination, analysis, and quantitative expression analysis, expression of FLAG-tagged or HA-tagged wild-type GC-A and of mutant GC-ADELTALys314-Gln330 and in HEK-293 cells |
| 4.6.1.2 | construction of a fluorescent alpha1/beta1-sGC heterodimer by fusion of the N-terminal YFP fragment and the C-terminal fragment to the N terminus and the C terminus of the alpha1- and beta1-subunit, respectively. Generated fusion proteins, wild-type and deletion mutants transiently expressed in a cGMP reporter cell line based on a Chinese hamster ovary cell line stably transfected with the cyclic nucleotide gated olfactory CNG2A-channel and cytosolic aequorin |
| 4.6.1.2 | construction of multiple N- and C-terminal deletion variants and cotransfecting them with full-length alpha1 subunit into COS cells |
| 4.6.1.2 | COS-7 cells transfected with the wild-type ONE-GC cDNA or its deletion mutants, ONE-GC mutants into pET30aLIC vector, expressed in Escherichia coli BL21-Codon-Plus-RIL |
| 4.6.1.2 | enzyme expression in heart tissue and heterogeneous expression patterns of NO-related regulatory enzyme systems, overview |
| 4.6.1.2 | expressed in Escherichia coli |
