EC Number |
---|
4.6.1.1 | - |
4.6.1.1 | AC5, RT-PCR expression analysis |
4.6.1.1 | AC9 |
4.6.1.1 | ACV expressed in Sf9 cells |
4.6.1.1 | ACV fused with a GST tag and expressed in Escherichia coli, wild-type and mutant ACVIdeltaN enzymes expressed in Sf9 cells, COS-7 cells overexpressing ACVI, binding domain vector pGBKT7 together with pGADT7-beta1 or the activating vector pGADT7 together with pGBKT7-N-terminal region of ACV or ACVI co-transformed into AH109 cells |
4.6.1.1 | catalytic domain and the holoenzyme expressed in Escherichia coli BL21(DE3)[pREP4] as soluble proteins |
4.6.1.1 | chimeras inserted into the mammalian expression vector pCMV-SK, wild-type and chimeric mutants expressed in HEK-293 cells |
4.6.1.1 | class I AC gene, overexpression of wild-type and mutant catalytic domain, residues 2-446, i.e. Cya2-446, in strain BL21(DE3) |
4.6.1.1 | cloned into pET15b vector, recombinant plasmid isolated from Escherichia coli NovaBlue cells and introduced into Escherichia coli BL21(DE3) cells for protein expression |
4.6.1.1 | cloning of AC7 cDNA from HEL cells |