EC Number |
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4.2.99.22 | expression in Escherichia coli |
4.2.99.22 | gene TgTCEA1, recombinant expression of the wild-type enzyme in Escherichia coli BL21-CodonPlus (DE3)-RIL/pET28a |
4.2.99.22 | gene TgTCEA1, sequence comparisons and unrooted phylogenetic tree, functional recombinant expression of the mature dimeric enzyme in Escherichia coli. Although the recombinant enzyme is functionally expressed as N-terminal His-tagged protein, removal of the vector-derived sequence including His-tag by protease treatment almost doubles its activity, allowing for accurate assessment of enzyme characteristics. Recombinant transient expression of GFP-tagged N-terminal transit peptides of TCEA in onion epidermal cell plastids |
4.2.99.22 | TCEA-b genes b1-b7 are higly homologous to each other, with six to seven single-nucleotide polymorphisms |