EC Number |
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4.2.3.39 | cloning from a cDNA library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue |
4.2.3.39 | expressed in Escherichia coli BL21(DE3) cells |
4.2.3.39 | functional expression in Saccharomyces cerevisiae, native mating type alpha yeast strain JBY574 and constructed strain EHY42, leading to production of sesquiterpenes in yeast. Expressing epi-cedrol synthase in the upc2-1 mutant CJ-2A actually decreases foreign sesquiterpene yields relative to wild-type strain. FPP is apparently less accessible to the epi-cedrol synthase in the upc2-1 mutant, overview. Mating type influences foreign sesquiterpene production, overview |
4.2.3.39 | homology-based cloning from a cDNA library, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strains DH5alpha and BL21(DE3), respectively |